STRUCTURE—FUNCTION RELATIONSHIPS OF NA+/K+‐PUMPS EXPRESSED INXENOPUSOOCYTES
作者:
WOLFGANG SCHWARZ,
LARISA A. VASILETS,
期刊:
Cell Biology International
(WILEY Available online 1996)
卷期:
Volume 20,
issue 1
页码: 67-72
ISSN:1065-6995
年代: 1996
DOI:10.1006/cbir.1996.0010
出版商: Blackwell Publishing Ltd
关键词: sodium pump;current—voltage dependence;cation binding mutation;regulation
数据来源: WILEY
摘要:
AbstractDuring the last years we have examined structure—function relationships in the Na+/K+‐ATPase with respect to interactions of the external cations with the pump molecule. We have analysed in voltage‐clamp experiments the influence of extracellular Na+and K+on the current generated by Na+/K+‐pumps expressed inXenopusoocytes. Our results demonstrated that external Na+and K+have to pass an access channel in the electrical field of the membrane to reach their binding sites. This external access, therefore, is voltage‐dependent and is affected by lysine residues within the cytoplasmic N‐terminus, by glutamic acid residues in intramembraneous domains, the ouabain sensitivity and phosphorylation by prot
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