Chloroplast DNABamHI restriction fragments were studied inPinuselliottiiEngelm. var.elliottii(11 trees),P.elliottiivar.densa(2 trees), the three taxonomic varieties ofPinuscaribaeaMorelet (39 trees), andPinusoocarpaSchiede (9 trees). Polymorphism was detected within bothP.elliottiivarieties, but not withinP.oocarpa. Each of theP.caribaeavarieties was monomorphic, but the Cuban variety,P.caribaeavar.caribaea, differed clearly from the Bahamian (P.caribaeavar.bahamensis) and Honduran (P.caribaeavar.hondurensis) varieties. Varietycaribaeashared the most frequent chloroplast genotype ofP.elliottiivar.elliottii, while varietiesbahamensisandhondurensisshared the second most frequent genotype ofP.elliottiivar.elliottii. TheP.oocarpachloroplast genotype was not found in other taxa of the present study, but was found previously inPinusechinataMill, andPinuspalustrisMill. The results of this study, taken together with other chloroplast data from subsectionAustrales, suggest a close relationship betweenP.caribaeaandP.elliottii. This confirms previous morphological and biochemical evidence, but several discrepancies were apparent and are discussed.