A Method for Histological Examination of Undecalcified Teeth
作者:
CrespiRoberto,
GrossiSara G.,
期刊:
Biotechnic&Histochemistry
(Taylor Available online 1992)
卷期:
Volume 67,
issue 4
页码: 202-206
ISSN:1052-0295
年代: 1992
DOI:10.3109/10520299209110067
出版商: Taylor&Francis
关键词: dental tissue;ground section;histological examination;mineralized tissue;phase-contrast microscopy;undecalcified teeth
数据来源: Taylor
摘要:
A method is presented for histological examination of undecalcified ground sections of tooth roots affected with periodontal disease. The roots were placed in Karnovsky's fixative overnight, postfixed in 2% buffered osmic acid, and dehydrated in an ascending series of ethanol. The specimens were then infiltrated with propylene-oxide and Epon-Araldite resin, embedded in Epon-Araldite, and sections were prepared using a cutting and grinding system. The resulting ground sections were 8-12μm thick. The sections were allowed to air dry at room temperature. When thoroughly dried, a coverglass was applied using resinous mounting medium DPX. The specimens were examined by phase-contrast microscopy. The method is useful for simultaneous examination of mineralized dental tissue and bacterial morphotypes covering the root surface of teeth involved with periodontal disease.
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