Encapsulation of mammalian cell with chitosan‐CMC capsule
作者:
Toshimitsu Yoshioka,
Ryogo Hirano,
Toshiaki Shioya,
Masatoshi Kako,
期刊:
Biotechnology and Bioengineering
(WILEY Available online 1990)
卷期:
Volume 35,
issue 1
页码: 66-72
ISSN:0006-3592
年代: 1990
DOI:10.1002/bit.260350110
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractViable hybridoma cells were encapsulated. The capsules were formed in one step by placing a drop of cell suspension mixed with negatively charged carboxymethylcellulose (CMC) into a positively charged chitosan solution through the interpolymeric ionic interaction between two oppositely charged polymers. These capsules were found to have a mean diameter of about 1. 5 mm and wall thickness of 3 μm. The cells grew in the capsules using supplemented DMEM/F12 (four kinds of growth factor). The maximum cell density in encapsulating cell culture reached 1 × 107cells/ml, 10 times higher than that obtained in the free cell culture. The maximum monoclonal antibody concentration in the free cell culture was 15μg/mL, but that in the capsule was 45μg/mL The antibody produced by the cell was concentrated about four times higher inside than outside of the capsu
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