Interaction of theBacillus subtilisspore protoplast, cortex, ion‐exchange and coatless forms with glutaraldehyde
作者:
S.P. Gorman,
E.M. Scott,
E.P. Hutchinson,
期刊:
Journal of Applied Bacteriology
(WILEY Available online 1984)
卷期:
Volume 56,
issue 1
页码: 95-102
ISSN:0021-8847
年代: 1984
DOI:10.1111/j.1365-2672.1984.tb04699.x
出版商: Blackwell Publishing Ltd
数据来源: WILEY
摘要:
Gorman, S.P. Scott, E.M. Hutchinson, E.P. 1984. Interaction of theBacillus subtilisspore protoplast, cortex, ion‐exchange and coatless forms with glutaraldehyde.Journal of Applied Bacteriology56, 95–102.Bacillus subtilisspores with altered ionic content were tested for their susceptibility to lysis with lysozyme or sodium nitrite following treatment with glutaraldehyde. The Ca‐form was more sensitive to glutaraldehyde (pH 4.0.and pH 7.9) than the untreated or H‐form. Removal of spore coat dramatically increased sensitivity of the spore to glutaraldehyde. Pretreatment of spores, the coats of which had been extensively removed, with glutaraldehyde (pH 7.9) reduced the rate of lysis by lysozyme and by sodium nitrite, whereas glutaraldehyde at pH 4.0.had little effect. Glutaraldehyde pretreatment (pH 4.0 and pH 7.9) reduced the amount of hexosamine released by lysozyme but not by nitrite from isolated cortical fragments. Spore protoplasts were more susceptible to 0.01% (w/v) glutaraldehyde at pH 4.0 and isolated spore coats adsorbed alkaline glutaraldehyde more rapidly. These results are discussed in terms of a possible mode of action of glutaraldehyde on the bacteria
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