Active polysomes in the axoplasm of the squid giant axon
作者:
Antonio Giuditta,
E. Menichini,
C. Perrone Capano,
M. Langella,
R. Martin,
E. Castigli,
B. B. Kaplan,
期刊:
Journal of Neuroscience Research
(WILEY Available online 1991)
卷期:
Volume 28,
issue 1
页码: 18-28
ISSN:0360-4012
年代: 1991
DOI:10.1002/jnr.490280103
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: axon;protein synthesis;mRNA;neurofilament protein
数据来源: WILEY
摘要:
AbstractAxons and axon terminals are widely believed to lack the capacity to synthesize proteins, relying instead on the delivery of proteins made in the perikaryon. In agreement with this view, axoplasmic proteins synthesized by the isolated giant axon of the squid are believed to derive entirely from periaxonal glial cells. However, squid axoplusm is known to contain the requisite components of an extra‐mitocliondrial protein synthetic system, including protdn factors, tRNAs, rRNAs, and a heterogeneous family of mRNAs. Hence, the giant axon could, in principle, maintain an endogenous protein synthetic capacity. Here, we report that the squid giant axon also contains active polysomes and niRNA, which hybridizes to a riboprobe encoding murine neurofilament protein. Taken together, these findings provide direct evidence that proteins (including the putative neuron‐specific neurofilament protein) are also synthesized de novo in the axonal compartm
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