Antibody to histo‐blood group A antigen neutralizes HIV produced by lymphocytes from blood group A donors but not from blood group B or O donors
作者:
Maiken Arendrup,
John-Erik Hansen,
Henrik Clausen,
Claus Nielsen,
Lars Mathiesen,
Jens Nielsen,
期刊:
AIDS
(OVID Available online 1991)
卷期:
Volume 5,
issue 4
页码: 441-444
ISSN:0269-9370
年代: 1991
出版商: OVID
关键词: HIV;neutralization;monoclonal antibody;carbohydrate epitopes;glycosylation;isolate-specific;host-cell-specific;blood group A antigen
数据来源: OVID
摘要:
Three virus isolates HTLV-IIIB/lyA, HTLV-IIIB/lyB and HTLV- IIIB/lyO, obtained by passaging and propagating the HTLV- IIIB/H9 isolate in three separate cultures of mixed peripheral blood mononuclear cells (PBMC) from donors of blood type A, B or O, respectively, were tested for susceptibility for virus neutralization by the monoclonal antibody (MAb) AH16 directed against the blood group A epitope. MAb AH16 was previously shown to inhibit cell-free virus infection using HTLV-IIIBpropagated in H9 cells. AH16 showed a concentration-dependent inhibition of the HTLV- IIIB/lyA isolate but did not inhibit the HTLV- IIIB/lyB or the HTLV- IIIB/lyO isolate. Specificity of the MAb-mediated inhibition was shown using A-antigen (tetrasaccharide). Thus, HIV infection of PBMC from donors with blood type A appears to induce expression of host-cell-encoded carbohydrate blood group A epitope on HIV which can be a target for MAb-mediated virus neutralization.
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