Ten cultivars of carnation were assessed for their ability to regenerate plants via direct organogenesis. As explants, leaf base of in vitro grown plants and flower buds from green house plants were used. Adventitious shoots apeared after 14 days of cultivation in induction media supplemented with (6-benzylaminopurin and 1-Naphthalenacetic acid). It was proved that the rate of the competence for organogenesis related to the genotype, phytohormone balance in culture media, season and the position of the leaf pairs on the donor plant used as a sourse of explant. The screened cultivars showed different regeneration capacity. Medium with 0.9 mg/l BAP and 0.3 mg/l NAA was suitable for most of the tested plant material. Using petal as an initial explant 8 of the 10 cultivars produced shoots. Regenerants of two cultivars were planted in soil but they showed premature flowering at greenhouse conditions. High regenerable genotype (Nasslada-DR) was selected.