Renal Phospholipase C and Diglyceride Lipase Activity in Spontaneously Hypertensive Rats
作者:
HIDEAKI,
KAWAGUCHI HIROSHI,
OKAMOTO HIDEYA,
SAITO HISAKAZU,
期刊:
Hypertension
(OVID Available online 1987)
卷期:
Volume 10,
issue 1
页码: 100-106
ISSN:0194-911X
年代: 1987
出版商: OVID
关键词: phospholipase C;phosphatidylinositol;prostaglandin E2;arachidonic acid;diglyceride lipase;phospholipids
数据来源: OVID
摘要:
Phospholipase C activity and diglyceride lipase activity were studied in the renal cortex and medulla of 10- and 40-week-old stroke-prone spontaneously hypertensive rats (SHRSP) and agematched normotensive Wistar-Kyoto rats (WKY). Enhanced phospholipase C activity was found in the cortical and medullary cytosol of kidney from SHRSP, and microsomal diglyceride lipase in SHRSP also increased. In SHRSP, phospholipase C and diglyceride lipase activities increased with age, but this increase was not evident in WKY. Phospholipase C had high substrate specificity for phosphatidylinositol in renal cytosol of both WKY and SHRSP. The increased activities were accompanied by prostaglandin E2synthesis in renal medullary microsomes of 10-week-old SHRSP and were also present in the kidney of 40-week-old SHRSP. Total phospholipid and arachidonic acid contents in kidney were markedly high in the medulla of 10-week-old SHRSP, but these lipids were decreased in 40-week-old SHRSP. These results suggest that phospholipids and arachidonic acid in SHRSP may be genetically high and that the activated phospholipase C and diglyceride lipase hydrolyze phospholipids, providing arachidonic acid for prostaglandin synthesis, which results in a decrease of phospholipids and arachidonic acid in the kidney of 40-week-old SHRSP. These studies demonstrate that a phosphatidylinositol-specific phospholipase C-prostaglandin synthetic system may play an important role in the course of hypertension in SHRSP.
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