Human IgG Antibodies to Carbohydrate and Protein Antigens in Mouse Protection Tests with Group B Streptococci
作者:
KAREN CHRISTENSEN,
POUL CHRISTENSEN,
GABRIEL DUC,
WALTER HITZIG,
VIVEKA LINDEN,
BEAT MULLER,
REINHARD SEGER,
期刊:
Pediatric Research
(OVID Available online 1984)
卷期:
Volume 18,
issue 5
页码: 478-482
ISSN:0031-3998
年代: 1984
出版商: OVID
关键词: ELISA, enzyme-linked immunosorbent assay;GBS, group B streptococci;CFU, colony forming units;cpm, counts per minute
数据来源: OVID
摘要:
SummaryThe protective effect of four commercial human gammaglobulin batches (I-IV) in mice was studied using six different strains of group B streptococci (GBS): types la; Ib; II, R-protein negative (R-); II, R+; HI, R-; and III, R+. Each mouse received 1.0 ml gammaglobulin and 0.5 ml bacteria, 106-108colony forming units (CFU). There was a close correlation between antibody levels measured by the use of radiolabeled protein A and the mouse-protective effect of the gamma-globulins. The mouse-protection tests demonstrated that batch I protected against GBS types la and III, R— at low concentration (65 mg/kg mouse weight), against type Ib at medium (260 mg/kg) and against type III, R+ at high concentration. Batch IV protected against types la and Ib, although the doses were four times higher than those in batch I, but did not protect against type III, R+. There was no mouse protection by any of the batches against type II. Antibody levels against Ibc and R, protein antigens, were substantially lower in batch IV. Because the results of these mouse-protection studies indicate the importance of such antibodies against protein antigens, batches I—III might be more useful for therapy of neonatal GBS-septicemia.
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