Four systems used for chemiluminescence determination of horseradish peroxidase[luminol-H2O2(NaOH), pyrogallol-H2O2(pH 6.5), luminol-H2O2-para-iodophenol (pH 8.5) and luminol-H2O2-para-hydroxylbiphenyl (pH 8.5)] were studied. The experimental conditions were optimized and linear dynamic ranges and limits of detection determined. The HRP conjugates with different antibodies were also determined, on the basis of which methods for determination of insulin, gentamycin and Salmonella anatis by chemiluminescent immunoassay were established.