Electrophoretic Studies on Molecular Defects of von Willebrand Factor and Platelet Glycoprotein llb-llla with Antibodies Produced in Egg Yolk from Laying Hens
作者:
F. Toti,
C. Gachet,
P. Ohlmann,
A. Stierlé,
L. Grunebaum,
M.-L. Wiesel,
J.-P. Cazenave,
期刊:
Pathophysiology of Haemostasis and Thrombosis
(Karger Available online 1992)
卷期:
Volume 22,
issue 1
页码: 32-40
ISSN:1424-8832
年代: 1992
DOI:10.1159/000216289
出版商: S. Karger AG
关键词: Immunoelectrophoresis;Yolk immunoglobulins;von Willebrand factor;Glycoprotein GP IIb-IIIa;Flow cytometry
数据来源: Karger
摘要:
Immunoglobulins isolated from egg yolk (IgYs) are a convenient source of polyclonal antibodies. Their purification is simple and the yields important (50 mg immunoglobulins/egg). Nevertheless their biochemical characteristics are different from those of rabbit antibodies generally used for the study of molecular defects of plasmatic and platelet proteins. Provided standardization is achieved, IgYs can be used for immunoelectrophoresis, immunoprecipitation or immunoblotting assays. We describe here the electrophoretic and immunoblotting conditions employed to explore human plasmatic and platelet von Willebrand factor (vWF) and human platelet GP Ilb-IHa using IgYs. These two proteins are involved in primary hemostasis and their absence or abnormality is responsible for hereditary bleeding disorders. The methods were applied to the characterization of patients with vWF or GP Ilb-IIIa defects and compared to classical mammalian IgG immunoelectrophoretic techniques. Results were further confirmed by flow cytometric analysis.
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