Donor-Derived b2a2-Specific T Cells for Immunotherapy of Patients with Chronic Myeloid Leukemia
作者:
Tania Crough,
Mie Nieda,
James Morton,
John Bashford,
Simon Durrant,
Andrew Nicol,
期刊:
Journal of Immunotherapy
(OVID Available online 2002)
卷期:
Volume 25,
issue 6
页码: 469-475
ISSN:1524-9557
年代: 2002
出版商: OVID
关键词: b2a2;Chronic myeloid leukemia;Dendritic cell;Immunotherapy;T cells
数据来源: OVID
摘要:
The BCR-ABL fusion proteins, b2a2 and b3a2, are potential targets for a beneficial graft-versus-leukemia (GVL) effect after allogeneic stem cell transplantation for chronic myeloid leukemia (CML). This study demonstrates that CD4+T cells specific to the b2a2 peptide can be generated from a normal allogeneic stem cell transplant donor after stimulation with monocyte-derived dendritic cells (Mo-DC) using culture conditions applicable to clinical use. Stimulation of donor T-cell enriched mononuclear cells (MNC) with b2a2-pulsed Mo-DC produced approximately 3 × 109b2a2-specific CD4+T cells. The CD4+T cells were HLA-DR7 restricted. These results confirm that the generation of donor derived b2a2-specific T cells for clinical use is feasible and warrants clinical testing after stem cell transplantation.
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