Nitric oxide reduces hydrogen peroxide production from human polymorphonuclear neutrophils
作者:
T. FORSLUND,
T. SUNDQVIST,
期刊:
European Journal of Clinical Investigation
(WILEY Available online 1995)
卷期:
Volume 25,
issue 1
页码: 9-14
ISSN:0014-2972
年代: 1995
DOI:10.1111/j.1365-2362.1995.tb01518.x
出版商: Blackwell Publishing Ltd
关键词: Chemiluminescence;collagen;nitroprusside;N‐ethylmaleimide;respiratory burst
数据来源: WILEY
摘要:
Abstract.Nitric oxide has been reported to affect both adhesion and respiratory burst of neutrophils. This indicates a possible role of nitric oxide in regulation of acute inflammatory responses. Release of oxygen metabolites from neutrophils can be measured using luminol‐enhanced chemiluminescence and this method can detect both extracellularly and intracellu‐larly released oxygen metabolites. Neutrophils treated with nitroprusside and activated with FMLP, type I collagen or PMA decreased their extracellular release of oxygen metabolites, while their intracellular release was almost unaffected. The effect of nitroprusside was mediated by nitric oxide since treatment with cyanide had the opposite effect. N‐ethylmaleimide treatment decreased both extra‐ and intracellular release of oxygen metabolites. This indicates that nitric oxide affects membrane‐bound NADPH‐oxidase either indirectly or directly, and not a cytosol factor of the oxidase as earlier shown for N‐ethylmaleimide. In conclusion, extracellular nitric oxide attenuates extracellularly released oxygen metabolites from activated neutrophils in an inflamma
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