The photosensitized oxidation of the calf lens main intrinsic protein (MP26) with hematoporphyrin
作者:
RobertsJ. E.,
RoyD.,
DillonJ.,
期刊:
Current Eye Research
(Taylor Available online 1985)
卷期:
Volume 4,
issue 3
页码: 181-185
ISSN:0271-3683
年代: 1985
DOI:10.3109/02713688509000848
出版商: Taylor&Francis
数据来源: Taylor
摘要:
Hematoporphyrin (HP), a drug used for the treatment of tumors including intraocular tumors, is an efficient photosensitizer. In addition to its therapeutic value, it also produces a phototoxic side effect in the skin. To test whether such effects may also occur in the eye, calf lens fiber membranes were photolyzed in the presence and absence of 1 mM HP. A marked increase (ca 5 times) in the photopolymerization of the calf lens membrane main intrinsic protein (MP26) was found in the presence of HP. Tenfold increases in destruction rates were found in losses of histidine.The MP26 was also photolyzed after tryptic and chymotryptic digestion to MP21, this resulted in an increased photopolymerization in the presence of 1 mM HP. These data suggest an age related increase in sensitivity of the lens fiber membrane proteins to such photoprocesses. The addition of both azide and penicillamine reduces the photosensitized loss of the main intrinsic protein.
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