Automated analysis of reticulocytes using fluorescent staining with both acridine orange and an immunofluorescence technique
作者:
Paul A. Seligman,
Robert H. Allen,
Stefan J. Kirchanski,
Peter J. Natale,
期刊:
American Journal of Hematology
(WILEY Available online 1983)
卷期:
Volume 14,
issue 1
页码: 57-66
ISSN:0361-8609
年代: 1983
DOI:10.1002/ajh.2830140107
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: reticulocytes;erythropoiesis
数据来源: WILEY
摘要:
AbstractWe have used an automated system to measure serial reticulocyte samples from phlebotomized rats and rats treated with the chemotherapeutic agent actinomycin‐D. In performing the automated samples, we utilized two different methods. The first method employed a fluorescent dye, acridine orange, which stains RNA in a manner similar to supravital stains presently used in performing reticulocyte counts. The second method is unique in that we utilized an immunofluorescent assay with antitransferrin recptor antibody which identifies the transferrin receptor found on the cell surface of reticulocytes but not on mature red cells. Both methods gave reproducible values in estimating the increase in reticulocytes seen after phlebotomy and the initial reticulocytopenia followed by recovery of the peripheral blood reticulocyte count seen after treatment with actinomycin‐D. Also, since the antitransferrin receptor antibody appears to recognize the more immature reticulocytes, both methods taken together may provide an estimate of the maturity of a population of reticulocy
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