In vivodetermination of fibril orientation in plant cell walls with polarization CSLM
作者:
J.‐P. VERBELEN,
D. STICKENS,
期刊:
Journal of Microscopy
(WILEY Available online 1995)
卷期:
Volume 177,
issue 1
页码: 1-6
ISSN:0022-2720
年代: 1995
DOI:10.1111/j.1365-2818.1995.tb03528.x
出版商: Blackwell Publishing Ltd
关键词: Cell wall;cellulose fibrils;Congo Red;fluorescence microscopy;Nicotiana;polarization CSLM
数据来源: WILEY
摘要:
SummaryCongo Red fluorescence is used to detect cellulose in the wall of plant cells. The orientation of the cellulose fibrils is determined by using polarized light for excitation. The absorption characteristics of Congo Red make this approach a method of choice for applications with any standard confocal scanning laser microscope (CSLM). The semiquantitative character of CSLM observations combined with the non‐toxicity of the stain allow a very fast and reliable assessment of cellulose orientation in the wall of living plant cell
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