THE LOCALISATION OF SORBITOL PATHWAY ACTIVITY IN THE RAT RENAL CORTEX AND ITS RELATIONSHIP TO THE PATHOGENESIS OF THE RENAL COMPLICATIONS OF DIABETES MELLITUS
作者:
JC Hutton,
PJ Schofield,
JF Williams,
FC Hollows,
期刊:
Australian Journal of Experimental Biology and Medical Science
(WILEY Available online 1975)
卷期:
Volume 53,
issue 1
页码: 49-57
ISSN:0004-945X
年代: 1975
DOI:10.1038/icb.1975.5
出版商: Nature Publishing Group
数据来源: WILEY
摘要:
SummaryA series ofin vivoandin vitroinvestigations was performed to examine the localisation of sorbitol pathway activity in the rat renal cortex and to investigate the possible relation that the accumulation of sorbitol pathway intermediates in renal cortical tissue may have to the pathogenesis of renal complications in diabetes mellitus.Neither of the sorbitol pathway intermediates, sorbitol or fructose, were detected either in intact glomeruli which had been isolated from rats rendered chronically diabetic with streptozotocin, or in metabolically active glomeruli which had been incubatedin vitroin high glucose media. Such data agreed with previously published observations that the enzyme aldose reductase is not present in renal glomeruli, and suggested that changes in sorbitol pathway activity cannot be directly related to the pathogenesis of diabetic glomerulosclerosis.Sorbitol was detected in low concentrations (3·1 μmol/g protein) in cortical tubules which had been isolated from the renal cortex of rats rendered chronically diabetic with streptozotocin. This concentration of sorbitol was higher than that in the intact renal cortex of the diabetic animal (0·3 μmol/g protein) or in the cortical tubules of non‐diabetic animals (0·5 μmol/g protein). It is apparent that the renal cortical tubule is a major site of sorbitol pathway activity in the renal cortex. However, there is presently no obvious causal relationship between the accumulation of such relatively low concentrations of sorbitol in the renal cortical tubule and the pathogenesis of glomerulosclerosis or cortical tubular lesions in diabetes.
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