Atrial and Brain Natriuretic Peptides Inhibit the Endothelin‐1 Secretory Response to Angiotensin II in Porcine Aorta
作者:
Masakazu Kohno,
Koji Yokokawa,
Takeshi Horio,
Kenichi Yasunari,
Koh-ichi Murakawa,
Tadanao Takeda,
期刊:
Circulation Research
(OVID Available online 1992)
卷期:
Volume 70,
issue 2
页码: 241-247
ISSN:0009-7330
年代: 1992
出版商: OVID
关键词: atrial natriuretic peptide;brain natriuretic peptide;endothelin;angiotensin II;aorta;pig
数据来源: OVID
摘要:
We have recently shown that the porcine aorta releases immunoreactive endothelin-1 in a time-dependent way. Here, we examined the inhibition by atrial natriuretic peptide (ANP) and brain natriuretic peptide (BNP) of endothelin-1 secretion after stimulation with angiotensin II (Ang II) by using porcine aorta. Ang II dose-dependently stimulated immunoreactive endothelin-1 secretion. Porcine ANP-(1–28) and porcine BNP-26 both inhibited such secretion in a dose-dependent way. The addition of a cyclic guanosine 5'-monophosphate (cGMP) analogue, 8-bromo-cGMP, reduced the immunoreactive endothelin-1 secretion after stimulation with Ang II. In cultured porcine endothelial cells the inhibition by porcine ANP-(1–28) and porcine BNP-26 of immunoreactive endothelin-1 secretion after stimulation with Ang II was paralleled by an increase in the cellular cGMP level. Rat ANP-(5–25) was weaker than porcine ANP-(1–28) in inhibiting immunoreactive endothelin-1 secretion and increasing cGMP in cultured cells. There was negative correlation between the percent decrease in immunoreactive endothelin-1 and the percent increase in cGMP. Neither porcine ANP-(1–28) nor BNP-26 affected the number or sensitivity of Ang II binding sites in cultured porcine endothelial cells. These results suggest that ANP and BNP inhibit endothelin-1 secretion after stimulation with Ang II, probably through a cGMP-dependent process.
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