Shoot regeneration from leaf discs ofLimonium perigrinumusing thidiazuron
作者:
JohnF. Seelye,
DebraJ. Maddocks,
GarryK. Burge,
EdR. Morgan,
期刊:
New Zealand Journal of Crop and Horticultural Science
(Taylor Available online 1994)
卷期:
Volume 22,
issue 1
页码: 23-29
ISSN:0114-0671
年代: 1994
DOI:10.1080/01140671.1994.9513802
出版商: Taylor & Francis Group
关键词: Limonium;regeneration;thidiazuron;transformation;tissue culture;pulsing
数据来源: Taylor
摘要:
Shoots were regenerated from 12‐mm diameter leaf discs derived from greenhouse plantsof Limonium perigrinum(Bergius), with either zeatin or thidiazuron (TDZ), the latter producing five times the number of shoots. After 8 weeks exposure to a modified Murashige and Skoog basal medium (BM) supplemented with 3.0 mg/litre TDZ, an average of 13 shoots/disc formed, 90% of which were ≤ 1 mm long. There was no significant difference in the total number of shoots formed at 1.0–5.0 mg/litre TDZ, however at lower rates (1.0–2.0 mg/litre) a greater proportion of longer shoots (> 1 mm) developed. Shoots regenerated after 8 weeks from leaf discs exposed to BM with TDZ for from 2 h to 6 weeks before being transferred to growth‐regulator‐free medium. A mean of 4.8 shoots/disc formed from the shortest TDZ pulse period, with the majority being longer than 1 mm. Serial sections of discs pulsed with TDZ medium showed many suppressed shoot initials within the new tissue originating from the cut edge of the discs.
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