Analytical techniques for the analysis of 1-, 2-, 3- and 4-nitrofluorene were developed to enable these species to be determined in the gas and particle phases of the atmosphere. The nitrofluorene isomers were synthesized by electrophilic and gas-phase OH radical-initiated nitration of fluorene and identified by their1H-NMR and mass spectra. For quantitative analysis deuterated 2- and 4-nitrofluorene were also synthesized for use as internal standard compounds. The GC retention indices of all six compounds were determined on DB-5, DB-1701 and SB-Smectic columns.