abstract–Seven methods for quantitative protein analysis were evaluated for the analysis of protein in saliva. The degree of interference from dialyzable components and from components retarded on Sephadex G‐50 was determined. Parotid saliva protein was fractionated into a glycoprotein‐rich and an amylase‐rich fraction on Sephadex G‐75, and the relative contributions from these protein fractions in the various protein tests were compared. Ultraviolet absorption at 215 mμ was recommended for the determination of total protein in saliva