Regulation of Cellular Retinoic Acid Binding Protein Expression in Rhino Mouse Skin by All-trans-Retinoic Acid
作者:
Gerard J. Gendimenico,
John P. Mallon,
Matthew A. Cromie,
James A. Mezick,
Anders Åström,
James T. Elder,
期刊:
Skin Pharmacology and Physiology
(Karger Available online 1995)
卷期:
Volume 8,
issue 4
页码: 167-172
ISSN:1660-5527
年代: 1995
DOI:10.1159/000211342
出版商: S. Karger AG
关键词: CRABP;All-trans-retinoic acid;Electrophoresis;mRNA
数据来源: Karger
摘要:
Cellular retinoic acid binding proteins (CRABP) are cytoplasmic proteins that bind all-trans-retinoic acid (RA) and other retinoids. The purpose of these studies was to determine the effects of topically applied RA on CRABP expression in rhino mouse skin. CRABP-II mRNA was significantly induced (3-to 4.5-fold) by a single dose of RA at 6 and 16 h after RA treatment, with a return to control levels at 48 h. CRABP-II message was not significantly elevated by 3 or 4 consecutive days of RA treatment, when assessed 24 h after the last treatment. CRABP-I mRNA was undetectable in control and RA-treated skin. We used radiolabelled RA binding combined with non-denaturing PAGE blot autoradiography to distinguish the CRABP subtypes. By this protein assay method, increases in CRABP-II were detected 24 and 48 h after a single application of RA, as well as after 3 and 4 days of RA treatment. RA treatment did not alter CRABP-I expression relative to the vehicle control. These results demonstrate that in mouse skin CRABP-II, but not CRABP-I, is inducible by RA, and is similar to how RA regulates CRABP in human skin.
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