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Production of monoclonal antibodies for detection of antigens in both their native and denatured states

 

作者: Vibeke Barkholt,   Kirsten Ebbehøj,   Susanne Sørensen,   Charlotte Arentsen,   Maria Dahl,   Hanne Frøklær,  

 

期刊: Food and Agricultural Immunology  （Taylor Available online 1994）
卷期: Volume 6, issue 2  

页码: 163-166

 

ISSN:0954-0105

 

年代: 1994

 

DOI:10.1080/09540109409354826

 

出版商: Taylor & Francis Group

 

关键词: Food analyses;denatured components;monoclonal antibodies;immunization procedures

 

数据来源: Taylor

 

摘要:

Different immunization procedures were tested to find methods to enhance the proportion of monoclonal antibodies reacting equally well with native and denatured proteins for use in food analysis. Antibodies to soybean trypsin inhibitor with the desired characteristics were obtained by immunization with the denatured protein. Antibodies to ovomucoid were obtained by long‐term immunization. The monoclonal antibodies to ovomucoid were of the IgM class.

 

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