Immunolocalization of Cytosolic Phytochrome in the Green AlgaMougeotia*
作者:
Christel Hanstein,
F. Grolig,
G. Wagner,
期刊:
Botanica Acta
(WILEY Available online 1992)
卷期:
Volume 105,
issue 1
页码: 55-62
ISSN:0932-8629
年代: 1992
DOI:10.1111/j.1438-8677.1992.tb00267.x
出版商: Blackwell Publishing Ltd
关键词: Chloroplast movement;immunoblot;immunofluorescence;Mougeotia, phytochrome
数据来源: WILEY
摘要:
AbstractThe monoclonal antibody Z‐3Bl (Schneider‐Poetsch et al., Planta 173 [1988] 61–72) was used to localize phytochrome in theMougeotiacell by indirect immunofluorescence. The antibody detected a protein of about 110 kD in the immunoblot. Immunofluorescence indicated abundant phytochrome throughout the cytoplasm. InMougeotia, a large cytoplasmic pool of phytochrome is presumably in equilibrium with a probably reactant‐bound Pfr‐pool at the plasmalemma, the latter being responsible for the physiological
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