Goldfish (Carassius carassius(L.) var.auratus) erythrocytes bound by antibodies against goldfish immunoglobulins
作者:
LuigiSalvatore Azzolina,
Cristina Fontanarosa,
Giuseppe Tridente,
期刊:
Bolletino di zoologia
(Taylor Available online 1987)
卷期:
Volume 54,
issue 1
页码: 55-58
ISSN:0373-4137
年代: 1987
DOI:10.1080/11250008709355556
出版商: Taylor & Francis Group
关键词: Agglutination;Erythrocytes;Fluorescence;Goldfish;Inhibition.
数据来源: Taylor
摘要:
We report that rabbit antibodies against goldfish immunoglobulins (C‐Ig) bind goldfish erythrocytes (C‐RBC) in agglutination and immunofluorescence assays. These reactions can be inhibited by absorption of rabbit antiserum with goldfish spleen leukocytes and either C‐Ig or their large fragments (> 40 kd M.W.) obtained after peptic digestion, but not with small fragments (< 20 kd). Trypsin treatment of C‐RBC enhances the agglutination titre of the rabbit antiserum even after 24 hrs incubation of trypsinized C‐RBC in tissue culture medium. The present data show that C‐RBC have membrane molecules which cross‐react with C‐Ig and therefore could be relevant in furthering our understanding of the biology of the nucleated RBC in lower vertebrates.
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