Histochemical studies onβ-glucuronidase activity in developing teeth and bone of rat and Macaque monkey
作者:
HasselgrenGunnar,
HammarströmLars E.,
期刊:
Acta Odontologica Scandinavica
(Taylor Available online 1975)
卷期:
Volume 33,
issue 3
页码: 161-167
ISSN:0001-6357
年代: 1975
DOI:10.3109/00016357509026357
出版商: Taylor&Francis
关键词: Glucuronidase;bone and bones;histochemistry;monkeys;rats;odontogenesis;tooth calcification
数据来源: Taylor
摘要:
The enzymeβ-glucuronidase (E.C. 3.2.1.31.) has been studied in developing teeth and bone of Sprague-Dawley rats and Macaque monkeys(Macaca Irus)by means of histochemistry using naphthol-AS-B1-β-D-glucuronide as substrate and hexazotized pararosanilin as coupling reagent. Sinceβ-glucuronidase is sensitive to fixatives a freeze-sectioning technique has been used which made it possible to section highly mineralizcd tissues without previous fixation and decalcification. In the bone enzyme activity was found in osteoclasts and osteoblasts.β-glucuronidase activity was found in teeth of both species in odontoblasts, ameloblasts, stratum intermedium, inner and outer enamel epithelium. When enamel matrix formation was completed as indicated by the reduction of ameloblast length the enzyme activity of the ameloblasts increased.
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