HIV‐1 Western blotdevelopment and assessment of testing to resolve indeterminate reactivity
作者:
David Healey,
William Maskill,
Teresa Howard,
Veronica Armstrong,
Wayne Bolton,
Gregory Cooper,
Jean Downie,
Lester Hiley,
Allison Imrie,
Sally Johnson,
Peter Robertson,
Sonia Schepetiuk,
期刊:
AIDS
(OVID Available online 1992)
卷期:
Volume 6,
issue 7
页码: 629-634
ISSN:0269-9370
年代: 1992
出版商: OVID
关键词: HIV-1;Western blot;indeterminate;testing strategy
数据来源: OVID
摘要:
ObjectiveTo reduce the number of HIV-1 Western blot (WB)-indeterminates requiring follow-up and the time taken to provide a clear positive or negative result.DesignIn the first of two stages, a testing and follow-up strategy was developed to resolve anti-HIV-1 status of WB-indeterminates. In the second stage, implementation of this strategy was assessed.MethodsAfter dividing indeterminates into four groups according to WB profile, samples were tested for anti-HIV-1, anti-HIV-2, anti-HTLV-1 antibodies, and HIV-1 antigen using the most sensitive assays available. When testing failed to clarify anti-HIV-1 status, follow-up samples were taken to monitor changes in antibody status.ResultsSamples in two out of the four indeterminate groups were negative for anti-HIV-1. The other two groups required additional testing and/or follow-up to distinguish reactivity caused by anti-HIV-1 from cross-reactivity.ConclusionGrouping HIV-1 WB-indeterminates according to profile allows a significant percentage to be reported as anti-HIV-1-negative, while additional testing may allow others to be reported as anti-HIV-1-positive. The remainder require a maximum of 3 months' follow-up to resolve anti-HIV-1 status.
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