Increased expression of 19-kD interacting protein-3-like protein and the relationship to apoptosis in the lung of rats with severe acute pancreatitis
作者:
Hayato,
Nakamura Hidekazu,
Honda Mitsuo,
Tashiro Masashi,
Taguchi Hiroyuki,
Yoshikawa Makoto,
期刊:
Critical Care Medicine
(OVID Available online 2003)
卷期:
Volume 31,
issue 10
页码: 2527-2534
ISSN:0090-3493
年代: 2003
出版商: OVID
关键词: experimental pancreatitis;acute lung injury;Bcl-2/E1B 19-kDa interacting protein-3-like protein;19-kD interacting protein-3-like protein;differential display
数据来源: OVID
摘要:
ObjectiveThe aim of the present study was to determine the underlying cellular mechanisms in the pancreas after acute pancreatitis and to study the pathogenesis of pancreatitis-associated lung injury. We applied a differential display analysis to normal pancreas and to the pancreas with acute pancreatitis in rats, and we examined the expression of the identified gene in the lung as well as the pancreas after acute pancreatitis.DesignControlled animal study.SettingResearch laboratory of an academic institution.SubjectsNinety male Wistar rats.InvestigationsPancreatitis was induced by retrograde intraductal infusion of 4% sodium taurocholate (100 &mgr;L/100 g of body weight). Data were compared with data from controls (sham).Measurements and Main ResultsWe cloned some expressed sequence tags and identified one complementary DNA fragment. The deduced protein was a polypeptide of 218 amino acids, which was almost identical to human 19-kD interacting protein-3-like (NIP3L) protein. The expression of rat NIP3L identified in this study increased slightly in the pancreas after induction of acute pancreatitis but showed a marked increase in the lung by both Northern and Western blot analysis. NIP3L immunoreactivity was noted in alveolar and epithelial cells of the control (sham) lung, and the immunoreactivity in these cells was elevated after induction of acute pancreatitis. Moreover, acute pancreatitis increased terminal deoxynucleotidyl transferase-mediated dUTP-biotin nick end labeling-positive alveolar and bronchiolar cells in the lung.ConclusionNIP3L may be involved in lung injury, which is one of the major causes of death in cases of severe acute pancreatitis.
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