Heteronuclear two‐dimensional15N‐ and13C‐nmr studies of DNA oligomers and their netropsin complexes using indirect proton detection
作者:
Joseph Ashcroft,
David H. Live,
Dinshaw J. Patel,
David Cowburn,
期刊:
Biopolymers
(WILEY Available online 1991)
卷期:
Volume 31,
issue 1
页码: 45-55
ISSN:0006-3525
年代: 1991
DOI:10.1002/bip.360310105
出版商: Wiley Subscription Services, Inc., A Wiley Company
数据来源: WILEY
摘要:
AbstractHeteronuclear multispin coherence proton‐detected two‐dimensional nmr spectroscopic experiments were used to obtain information on protonated carbons and nitrogens of the self‐complementary d (G‐G‐T‐A‐T‐A‐C‐C) and d (G‐G‐A‐A‐T‐T‐C‐C) duplexes, with and without the drug netropsin dissolved in aqueous solution. Many correlations of protons coupled to13C nuclei on the base and sugar rings of the octanucleotides were detected, allowing the carbon resonances to be assigned based on previous homonuclear proton two‐dimensional nmr studies. Imino nitrogen assignments can also be made using the proton assignments from previous one‐dimensional nuclear overhauser effect experiments. Imino nitrogen shifts may be useful indicators of changes in local hydrogen‐
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