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Improving Biological Dyes and Stains: Quality Testing Versus Standardization

 

作者: SchulteErik K. W.,  

 

期刊: Biotechnic&Histochemistry  (Taylor Available online 1994)
卷期: Volume 69, issue 1  

页码: 7-17

 

ISSN:1052-0295

 

年代: 1994

 

DOI:10.3109/10520299409106255

 

出版商: Taylor&Francis

 

关键词: dyes;image analysis;quality control;stains;standardization

 

数据来源: Taylor

 

摘要:

This paper discusses the impact of both standardization and quality testing of dyes and stains in biology and medicine. After a brief review of why standardized dyes and stains are not presently available commercially, two types of testing and ways of improving dye quality are described. National or international organizations could be established to define standardization of dyes and stains. Standardization would be specifically defined as a list of physico-chemical parameters such as elaborated in this paper. Commercial batches of comparable quality may be labeled by the supplier as“standard dye.”a procedure currently performed by the European Council for Clinical and Laboratory Standardization (ECCLS). Also recommended to improve dye quality is commercial dye testing by independent laboratories with subsequent certification for use. This sort of quality control is currently carried out in the United States by the Biological Stain Commission (BSC). The advantages and disadvantages of both techniques and the use of image analysis for the definition of standards are discussed. A combination of both the BSC testing protocols and the ECCLS standards should be established for extended quality control of biological dyes and stains.

 

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