Effects of pH and Buffer Systems on Resting Spore Germination of the Grasshopper (Orthoptera: Acrididae) Pathogen,Entomophaga calopteni(=Entomophaga grylli, Pathotype 2) (Entomophthorales: Entomophthoraceae)
作者:
W. D. Valovage,
R. S. Kosaraju,
期刊:
Environmental Entomology
(OUP Available online 1992)
卷期:
Volume 21,
issue 5
页码: 1202-1211
ISSN:0046-225X
年代: 1992
DOI:10.1093/ee/21.5.1202
出版商: Oxford University Press
关键词: Acrididae;Entomophthorales;Entomophaga
数据来源: OUP
摘要:
Initial resting spore germination for two isolates (RF 150 and RF 250) ofEntomophaga calopteni(Bessey) Humber [=Entomophaga grylli(Fresenius) Batko, pathotype 2], a fungal pathogen of grasshoppers (Acrididae), was recorded for five different buffer systems: orthophosphate (OPHO), citrate-phosphate (CPHO), tris (hydroxymethyl) aminomethane (THMM), glycine-NaOH (GLYC), and tris-maleate (TMAL). For each buffer system, eight pH levels were tested ranging from 4 to 11. GLYC at pH 7–9 supported the highest germination percentages compared with the other combinations tested. In general, highest germination levels for all buffer systems occurred in the pH range of 6–8, although they were skewed toward the basic side of the pH scale in OPHO, THMM, and GLYC for isolate RF 150. THMM supported germination better than any other buffer system under highly alkaline conditions. Soil pH may be an important factor in disease transmission under natural conditions. The combined effects of temperature, photoperiod, pH, and buffer medium should be considered when reporting and comparing spore germination levels.
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