Conformational differences in two mutant hinge IgG4 antibodies observed by scanning tunneling microscopy
作者:
K. M. Kreusel,
J. R. Adair,
N. R. A. Beeley,
M. C. Davies,
D. E. Jackson,
C. J. Roberts,
S. J. B. Tendler,
P. M. Williams,
期刊:
Journal of Vacuum Science&Technology B: Microelectronics and Nanometer Structures Processing, Measurement, and Phenomena
(AIP Available online 1994)
卷期:
Volume 12,
issue 3
页码: 1517-1520
ISSN:1071-1023
年代: 1994
DOI:10.1116/1.587276
出版商: American Vacuum Society
关键词: ANTIBODIES;STM;CONFORMATIONAL CHANGES;MUTANTS;IMMOBILIZATION;SPRAY COATING;PLATINUM;CARBON;TOPOGRAPHY
数据来源: AIP
摘要:
Images have been obtained of two types of chimeric B72.3 anti‐TAG‐72 mutant hinge IgG4 antibodies using scanning tunneling microscopy. The antibodies were specifically engineered to show differences in their conformation due to site specific modifications in their hinge regions. To overcome initial difficulties due to tip sweeping effects, the method of immobilization employed was spray deposition on mica followed by rotary shadowing with platinum/carbon (Pt/C). Topographic data were obtained, and differences between the two mutant forms of the antibody were clearly visible.
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