ObjectiveThe generalized sympathetic activation induced by exercise is accompanied by an increase in heart rate, blood pressure and vascular resistance in non-exercising vascular beds. The aim of the present study was to test the feasibility of assessing, non-invasively, the static and oscillatory pressure/flow relationships of peripheral arteries and their continuous changes during dynamic exercise.DesignWe studied 44 healthy humans at rest, during moderate exercise (recumbent bicycle exercise) and recovery using a totally non-invasive approach.MethodsArterial pressure was measured using a plethysmographic device, and ipsilateral brachial artery flow and palmar skin microcirculation flow were assessed with continuous wave Doppler and laser Doppler, respectively. Continuous, long data segments (> 90 s) were acquired with a personal computer and used to determine the changes of pressure/flow relationships of peripheral arteries during dynamic exercise. A new simplified method utilizing a transfer function analysis extracted automatically unequivocal indexes of static and oscillatory properties of vascular system.ResultsModerate exercise induced significant increases of the static (Z0) and oscillatory (Zc) components of peripheral pressure/flow relationships in both brachial artery and skin microcirculation beds. Both indexes returned to control values during early recovery.ConclusionsThis simple, non-invasive approach was capable of assessing the changes of static and oscillatory vascular properties induced by dynamic exercise. This method could be applied for a better understanding of the vascular modifications that occur in other physiological or pathophysiological conditions also characterized by increases in sympathetic drive.