Application of Linear Image Sensor Toi Immunoassay Based Oin Turbidimetry of Antibody-Bound Latex Beads
作者:
Hideaki Matsuoka,
Seiichi Tanioka,
Isao Karube,
期刊:
Analytical Letters
(Taylor Available online 1987)
卷期:
Volume 20,
issue 1
页码: 63-79
ISSN:0003-2719
年代: 1987
DOI:10.1080/00032718708082237
出版商: Taylor & Francis Group
数据来源: Taylor
摘要:
A linear image sensor combined with a microcomputer system was applied to the immunoassay for human immunoglobulin G (H-IgG) based on turbidimetry. Antibody bound latex beads (Ab-L) were reacted with H-IgG in a glass capillary (0.8mmΦ× 70mm). The light scattering intensity profilesalong the capillary were analyzed from the light intensities at each element of the image sensor. A good correlation was obtained between the normalized variation of light intensity at 15 min and the concentration of H-IgG in the range 5×10−85×10−10g.ml−1.
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