There has been much interest recently in central California for reusing drainage water to grow trees. A sand-culture study was conducted to investigate the accumulation of boron (B) and selenium (Se) in eight hybrid poplar (Populus) clones irrigated with synthetic agricultural effluent containing increasing levels of chloride salt, B, and Se. Electrical conductivity (EC) ranged from 1.5 to 15 dS m-1, B levels from 1 to 5 mg L-1, and Se levels from 100 to 500 μg L-1. Compared with all tree organs, the leaves accumulated the greatest concentrations of B and Se at the time of harvest. The results show that pooled leaf B concentrations were positively correlated with EC levels (r = 0.78, P < 0.001) and negatively correlated (r = -0.53, P < 0.001) with leaf dry matter for all clones at all tested B levels. Combined leaf and stem Se data show, respectively, a significant decrease (P < 0.05 level) in tissue accumulation of Se with increased salinity. Toxicity symptoms (e.g., burning leaf margins, shoot die back) occurred in most clones grown at 12 and 15 dS m-1treatments leading to leaf abscission. Based on the data, clone 49177 (Populus trichocarpa×P. deltoidus) best tolerated the tested parameters among the clones and accumulated the greatest amount of B and Se. The moderate ability of the Populus species to remove and accumulate B and Se from saline effluent is most effective at salinity levels less than 7 dS m-1.