SummaryPurpose. This study was to determine whether age-related decrements in type I collagen synthesis occur in human eyelid skin. Methods. Using an antibody to procollagen I, we investigated collagen synthetic activity in skin removed for cosmetic purposes from 10 white patients between the ages of 4 and 77 years. Eleven masked referees graded the immunostaining on a scale of 1 (most intense) to 10 (least intense). Results. The multiple range test for rank by group demonstrated more intense staining in younger patients compared with older patients. An average correlation coefficient of 0.8432 (p < 0.05) existed between each of the referee's rankings. Conclusion. Type I collagen synthesis diminishes with age in eyelid skin.