LC/ESI-MS Allows Simultaneous and Specific Quantification of SDZ RAD and Cyclosporine, Including Groups of Their Metabolites in Human Blood
作者:
Gabriele Kirchner,
Christian Vidal,
Michael Winkler,
Lueke Mueller,
Wolfgang Jacobsen,
Anke Franzke,
Karl-Friedrich Sewing,
期刊:
Therapeutic Drug Monitoring
(OVID Available online 1999)
卷期:
Volume 21,
issue 1
页码: 116-122
ISSN:0163-4356
年代: 1999
出版商: OVID
关键词: Analytics;Cyclosporine;ESI-MS;Immunosuppressants;Macrolides;Metabolites;SDZ RAD
数据来源: OVID
摘要:
An analytic technique using liquid chromatography (LC) coupled with electrospray-mass spectrometry (ESI-MS) has been developed for the simultaneous determination of the new immunosuppressant SDZ RAD (40-O-[2-hydroxy)ethyl-rapamycin) and cyclosporine (Cs), including their metabolites in blood. With the time-sparing, automated on-line extraction technique, the recovery of SDZ RAD averaged 95% and that of Cs, 94%. The calibration lines were linear from 0.5 to 100 µg/L (r2 = 0.99) for SDZ RAD and from 10 to 1,000 µg/L (r2 = 0.99) for Cs. The method has been tested on blood samples from renal transplant recipients taken between 1 and 5 hours after oral SDZ RAD and Cs administration. In blood, we found the following metabolites: Hydroxy-SDZ RAD, dihydroxy-SDZ RAD, demethyl-SDZ RAD, and the ring-opened form of SDZ RAD. The main metabolite of SDZ RAD in blood was hydroxy-SDZ RAD. This novel LC/ESI-MS technique provided an excellent method for simultaneous quantitative monitoring of SDZ RAD and Cs, including their relevant groups of metabolites in patients treated simultaneously with these immunosuppressants,
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