Continuous Amperometric Determination of Glucose Using an Immobilized Enzyme Reactor in Combination with an Immersible Dialysis Probe
作者:
CarlFredrik Mandenius,
期刊:
Analytical Letters
(Taylor Available online 1988)
卷期:
Volume 21,
issue 10
页码: 1817-1832
ISSN:0003-2719
年代: 1988
DOI:10.1080/00032718808066349
出版商: Taylor & Francis Group
关键词: Glucose Determination;In-Situ Dialysis Probe;Continuous Measurement;Amperometric Enzyme Electrode;Enzyme Reactor;Glucose Oxidase
数据来源: Taylor
摘要:
Glucose was continuously determined by reaction in a packed-bed enzyme reactor containing glucose oxidase and catalase. Oxygen consumption was measured amperometrically with a polarographic Clark electrode. Glucose was sampled through a dialysis probe immersed in the solution to be measured. An extension of the normal range for the enzyme was achieved by modulating the flow rate through the dialysis probe and a linear response was obtained in the range of 1.0-60 mM glucose. The correlation between the glucose transfer and the membrane area of the dialysis probe was also studied. Six different membranes were used, all showing variations in the adhesion of yeast cells.
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