We examined habitat-use patterns in a fish assemblage in a large warmwater stream in West Virginia. Fish species and life stage composition and densities differed among habitat types, and five habitat-use guilds (edge pool, middle pool, edge channel, riffle, and generalist) were proposed. Larger centrarchids used deep habitats with slow velocities, whereas young centrarchids used shallower habitat. Juvenile and adult smallmouth bassMicropterus dolomieuiwere nearly ubiquitous in the habitats of the study area, although densities were highest among snags. Minnows and darters used shallower areas, but the range of velocity used differed among species and life stages. Vegetated and channel edge habitats served as nursery areas. Total fish densities were highest in edge pool, backwaters, snags, edge riffles, and riffles. Nearshore, structurally complex habitats seem important in influencing the assemblage structure of fishes of large streams.