Restriction enzyme digestion analysis and direct human globin gene cloning have permitted analysis of the physical arrangement of nucleotide sequences within and surrounding the human globin genes. With these methods it has been shown that the linear arrangement 5′to 3′of the globin genes isGγ-Aγ-δ-β. TheGγandAγgenes are separated by about 3.5 kilobases (kb), while theAγandδgenes are 15 kb apart, and theδandβ6.5 kb apart. Each of these genes contains a large intervening sequence (IVS) of approximately l kb in precisely the same position between codons 104 and 105. In addition, each of these genes has a small IVS between codons 30 and 31.