Modification of the polyethylene glycol 6000 precipitation method for recovering human and indicator viruses from oysters and mussels
作者:
GillianD. Lewis,
Angela Hough,
DavidH. Green,
JohnE. Hay,
LynnetteR. Ferguson,
期刊:
New Zealand Journal of Marine and Freshwater Research
(Taylor Available online 1996)
卷期:
Volume 30,
issue 4
页码: 443-447
ISSN:0028-8330
年代: 1996
DOI:10.1080/00288330.1996.9516732
出版商: Taylor & Francis Group
关键词: human virus;virus;enterovirus;F RNA bacteriophage;shellfish;mussel;oyster
数据来源: Taylor
摘要:
Human viruses are a common contaminant of shellfish affected by human sewage wastes. They are difficult to detect as they are not easily separated from shellfish tissue. This paper describes a modification of the polyethylene glycol (PEG) precipitation technique for recovery of enteroviruses and F‐specific bacteriophages from the Pacific oyster (Crassostrea gigas) and the green‐lipped mussel (Perna canaliculus). Modifications adopted were the use of only the digestive gland tissue for virus extraction, resuspension of the primary PEG pellet in 4 volumes of eluent, and the introduction of a secondary PEG precipitation to reconcentrate the virus containing extract. The recovery rate of the virus extraction process was not affected by introduction of the secondary concentration step (overall recovery remained at 60–70% of the virus input). The advantages of reduction of tissue residue in the extract, smaller final volume, and the ability to process 2–3 times the number of individual shellfish for the same effort, improve the practicality of the method.
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