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Increased Number of Angiotensin II Binding Sites Determined by Autoradiography in Anterior Pituitary of Water-Deprived and Brattleboro Rats

 

作者: Anita Israel,   Laura Plunkett,   Juan M. Saavedra,  

 

期刊: Neuroendocrinology  (Karger Available online 1986)
卷期: Volume 42, issue 1  

页码: 57-63

 

ISSN:0028-3835

 

年代: 1986

 

DOI:10.1159/000124249

 

出版商: S. Karger AG

 

关键词: Angiotensin II receptors;Receptor autoradiography;Rat anterior pituitary;Diabetes insipidus;Dehydration;Vasopressin;Water deprivation

 

数据来源: Karger

 

摘要:

Pituitary angiotensin II (ANG) binding sites were characterized by autoradiography in individual male Long Evans (LE) rats, heterozygous Brattleboro (HZ) rats, and homozygous Brattleboro (DI) rats which were water satiated or water deprived. An additional group of DI rats was treated with arginine vasopressin for 1 week. The technique utilized 8-µm pituitary sections which were incubated with 125I-[Sar1]-ANG in concentrations ranging from 25 pM to 10 nM. Angiotensin binding in the anterior pituitary of water-satiated LE rats was characterized by a single class of high-affinity, saturable sites with a Bmax of 1.309 ±119 fmol/mg of protein and a Ka of 0.51 ± 0.03 × 109M–1l. Compared to LE rats the density of ANG-binding sites in anterior pituitary was higher in DI rats (+84.2%), with HZ having an intermediate concentration (+41.6). Dehydration increased the ANG-binding site density in all groups. Five days of water deprivation increased the number of ANG-binding sites by 86.5% and by 36.9% in LE and HZ rats, respectively, when compared to their water-satiated controls. After 1 day of water deprivation, the ANG-binding site density increased by 19.4% in DI rats. No changes in ANG-binding sites occurred after hormonal replacement with arginine vasopressin in water-satiated DI rats. The binding affinity constant of the agonist for the ANG-binding sites compared with LE rats was decreased in both HZ and DI rats (-27.5 and -19.4%) and was also decreased after 5 days of dehydration in LE rats (-34%) when compared to the water-satiated state. Our results demonstrate alterations in ANG-binding sites in the anterior pituitary gland of both dehydrated Brattleboro rats and LE rats. These findings suggest a role for anterior pituitary ANG receptors in the regulation of fluid metabolism in th

 

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