An Automated Spectrophotometric Flow Injection Assay of Alkaline Phosphatase
作者:
M. Masoom,
期刊:
Analytical Letters
(Taylor Available online 1988)
卷期:
Volume 21,
issue 12
页码: 2381-2388
ISSN:0003-2719
年代: 1988
DOI:10.1080/00032718808059916
出版商: Taylor & Francis Group
关键词: Automation;Flow injection analysis;Alkaline phosphatase
数据来源: Taylor
摘要:
Assay of alkaline phosphatase using an automated flow injection manifold is described. The detector used is a spectrophotometer. The measurement is based on the rate of formation of p-nitrophenol at 405 nm, from the substrate p-nitropheny1 phosphate. The linear range, precision and accuracy of the technique for the assay of the enzyme is given. The advantages of the approach to assay of plasma enzymes are also discussed.
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