Flow cytometric detection of platelet activation in patients undergoing diagnostic and interventional coronary angiography
作者:
VossR.,
ScarlatT.,
MatzdorffA.,
TillmannsH.,
期刊:
Platelets
(Taylor Available online 1996)
卷期:
Volume 7,
issue 4
页码: 237-241
ISSN:0953-7104
年代: 1996
DOI:10.3109/09537109609023584
出版商: Taylor&Francis
数据来源: Taylor
摘要:
In 30 patients we investigated the expression of activated GPIIb/IIIa-complex as an indicator ofin vivoplatelet activation before and after coronary angiography/angioplasty. Patients were divided into three groups according to aspirin treatment: group I: patients having a diagnostic coronary angiography without aspirin (n= 6); group II: patients having a diagnostic coronary angiography with low dose aspirin therapy (n= II); group III: patients having a coronary angioplasty with low dose aspirin therapy plus aspirin i.v. (n= 13). Platelets were identified in a flow cytometer by their characteristic light scatter profile and binding of anti-GP Ib, and activated platelets by an antibody to the activated GP IIb/IIIa. Cardiac catheterization lead to an increase of the mean anti-GP IIb/IIIa-fluorescence of the platelets and of the percentage of platelets with an anti-IIb/IIIa-FL exceeding a fixed threshold value (subpopulation). While aspirin substantially inhibited the increase induced by diagnostic angiography, the increase in the angioplasty group was the greatest of all groups despite aspirin.
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