Intensification of mass transfer in aqueous two‐phase systems
作者:
Sanjiv V. Save,
Vishwas G. Pangarkar,
S. Vasanth Kumar,
期刊:
Biotechnology and Bioengineering
(WILEY Available online 1993)
卷期:
Volume 41,
issue 1
页码: 72-78
ISSN:0006-3592
年代: 1993
DOI:10.1002/bit.260410110
出版商: Wiley Subscription Services, Inc., A Wiley Company
关键词: aphrons;aqueous two phase;enzyme;extraction;intensification
数据来源: WILEY
摘要:
AbstractA novel technique which intensifies conventional aqueous two‐phase extraction by conversion of dispersed phase into colloidal gas aphrons (CGAs) has been developed for extraction of an enzyme. In the present work, amyloglucosidase (1,4‐α‐D‐glucan glucohydrolase) was extracted using a polyethylene glycol‐sodium sulfate‐water system. The lighter phase, i.e., polyethylene glycol (PEG) rich phase, was converted into CGAs which were then dispersed into a salt rich phase. The effect of type of surfactant and its concentration, dispersed phase velocity, phase composition, and type of sparger on the dispersed phase mass transfer coefficient was investigated. The results suggests 9–16 times higher values of mass transfer coefficient compared to spray column. The multiorifice sparger at concentrations of 0.33 g/L of cetyl trimethyl ammonium chloride yielded best results. © 1993 John
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