Light exposure not only elicits a photic response but may also alter the metabolism and functional properties of the retina. This may be evoked by the stimulation of phospholipid derived second messenger systems. In this study, we investigated the light-evoked release of inositol 1,4,5-trisphosphate in the isolated rat retina in vitro by means of high performance liquid chromatography (HPLC) detection. After prelabelling of isolated retinae with tritiated myo-inositol in darkness, they were exposed to no light or to white fluorescent light of 10,000 lux illuminance for 3,5 and 10 sec, respectively. We observed a 200% increase in the release of inositol 1,4,5-trisphosphate compared to basal values in darkness after 3 sec of light exposure with a decline after 5 sec and a return to basal values after 10 sec indicating a rapid breakdown of inositol 1,4,5-trisphosphate. Our data confirm previous studies in the amphibian retina and photoreceptors and demonstrate for the first time a light evoked inositol 1,4,5-trisphosphate release in the mammalian retina.