METHODS OF PROTEIN EXTRACTION FROM NEUROSPORA CRASSA
作者:
G. J. Stine,
W. N. Strickland,
R. W. Barratt,
期刊:
Canadian Journal of Microbiology
(NRC Available online 1964)
卷期:
Volume 10,
issue 1
页码: 29-35
ISSN:0008-4166
年代: 1964
DOI:10.1139/m64-005
出版商: NRC Research Press
数据来源: NRC
摘要:
Nine methods for disrupting the mycelium ofNeurospora crassahave been compared. Protein percentages are calculated per gram dry weight of mycelium. A TPN-specific glutamic acid dehydrogenase was extracted and the efficiency of each extraction method is given as total enzyme extracted and specific activity. In terms of total protein, total enzyme, and practicality of the method, the Hughes Press, the French Press and the Raper–Hyatt Press were found to be the most efficient. The advantages and limitations of each method are considered.
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