Purpose.To determine the in vitro antifungal activity of voriconazole, a new triazole, compared with other polyene and imidazole antifungal agents against corneal isolates ofScedosporium apiospermum.Methods.Macro-broth dilution susceptibility testing was performed on five isolates ofS. apiospermumobtained from patients with keratomycosis to determine the minimal inhibitory concentrations (MICs) for amphotericin B, natamycin, ketoconazole, itraconazole, and voriconazole. The use of oral voriconazole in the management of a patient with posttraumaticS. apiospermumkeratitis is described.Results.S. apiospermumis generally resistant to commonly used topical ophthalmic antifungal agents. The MIC of voriconazole was 0.5 &mgr;g/mL, a concentration lower than that of other imidazoles.Conclusion.Voriconazole has promising activity againstS. apiospermumand may prove useful in the management of fungal keratitis.