首页   按字顺浏览 期刊浏览 卷期浏览 High Performance Liquid Chromatographic Assay of Methylphenobarbital Metabolites in Uri...
High Performance Liquid Chromatographic Assay of Methylphenobarbital Metabolites in Urine

 

作者: H.,   Kunze W.,   Hooper M.,  

 

期刊: Therapeutic Drug Monitoring  (OVID Available online 1981)
卷期: Volume 3, issue 1  

页码: 45-50

 

ISSN:0163-4356

 

年代: 1981

 

出版商: OVID

 

关键词: Barbiturate metabolites;High pressure liquid chromatography;Mepho-barbital

 

数据来源: OVID

 

摘要:

A reversed-phase liquid chromatographic procedure was developed for simultaneous quantitation of three metabolites of the anticonvulsant methylphenobarbital in urine. These werep-hydroxyphenobarbital, phenobarbital, andp-hydroxymethylphenobarbital. Enzymatic hydrolysis was employed for liberation of the phenolic barbiturates from their glucuronide conjugates. Two internal standards were used at widely different concentrations, which conferred on the assay an accuracy over a wide concentration range. Concentration and instrument response (ultraviolet absorption at 215 nm) were linearly related over the concentration ranges of interest. The within-batch and between-day coefficients of variation were less than 4% in all cases. Recovery of all three analytes from the urine was nearly complete, and no substances that interfered with the assay were encountered in clinical specimens.

 

点击下载:  PDF (370KB)



返 回